Optimization of Process Conditions of Cotton Fabric Dyeing with Nettle Leaf Extract for Antibacterial Application Using Central Composite Design

In this study, all conditions of the natural dyeing process cotton fabric with antibacterial efficiency, extracted from leaves nettle were optimized using a central composite design. The are: concentration 16.943 g, temperature 50 °C, time 40 min, and color strength obtained at optimum condition was 1.53. Nettle dye is applied to fabrics by Pad-Dry-Cure method for imparting properties textile product apparel applications. Biomordants on scoured in pre-mordanting dyeing. Color characteristics dyed measured CIE L*a*b* value spectrophotometer. Gram-positive (S. aureus) gram-negative (E. coli) bacterial assessments performed quantitatively AATCC-100 test method. results provided evidence that treated exhibits higher efficiency gram-positive bacteria 96.5–98% reduction compared control samples, respectively. one having highest activity washed 15 times under same samples taken after 1, 5, 10, washes. They retained nearly 91% S. aureus 94% E. coli up 10 launderings against two bacteria.